ADAMTS13 Activity

Single-analyte tests

• ADAMTS13 Activity 117913

  Plasma · Fasting not stated · Turnaround 1 - 2 days

  Method and collection

  Method

  In Labcorp’s liquid chromatography‐tandem mass spectrometry (LC/MS-MS) assay, ADAMTS13 activity is determined by measuring the cleavage of a synthetic polypeptide substrate (referred to as vWF73) added to plasma samples.6 The amino acid sequence of vWF73 corresponds to amino acid residues 1596 through 1668 of mature von Willebrand Factor (vWF) and, thereby, possesses the tyrosine‐methionine cleavage site and exosite necessary to undergo specific cleavage by plasma ADAMTS13.7 Using LC/MS-MS, cleavage of vWF73 is determined directly by measuring a specific proteolytic product of vWF73 whose quantity is directly proportional to the activity of ADAMTS13 in plasma. The LC/MS-MS activity assay is traceable to the WHO 1st International Standard for ADAMTS138 to ensure accurate measurements and offers several advantages relative to comparable fluorescence‐based assays.

  Collection

  Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Serum gel-barrier tubes and serum tubes with clot initiators should aslo be collected after the citrate tubes. Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). The specimen should be frozen immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested . Please see Shipping Procedure for Priority ADAMTS13 Activity for information on direct shipping procedures.

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• ADAMTS13 Activity Reflex Profile 117921

  Plasma · Fasting not stated · Turnaround 2 - 4 days

  Method and collection

  Method

  In Labcorp’s liquid chromatography‐tandem mass spectrometry (LC/MS-MS) assay, ADAMTS13 activity is determined by measuring the cleavage of a synthetic polypeptide substrate (referred to as vWF73) added to plasma samples.6 The amino acid sequence of vWF73 corresponds to amino acid residues 1596 through 1668 of mature von Willebrand Factor (vWF) and, thereby, possesses the tyrosine‐methionine cleavage site and exosite necessary to undergo specific cleavage by plasma ADAMTS13.7 Using LC/MS-MS, cleavage of vWF73 is determined directly by measuring a specific proteolytic product of vWF73 whose quantity is directly proportional to the activity of ADAMTS13 in plasma. The LC/MS-MS activity assay is traceable to the WHO 1st International Standard for ADAMTS138 to ensure accurate measurements and offers several advantages relative to comparable fluorescence‐based assays.

  Collection

  Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Serum gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a transfer pipette) carefully remove two-thirds of the plasma without disturbing the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). The specimen should be frozen immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

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Panels that include this biomarker

• COVID-19 Induced Thrombotic Microangiopathy Profile 164044

  6 analytes · Serum · Fasting not stated · Turnaround 2 - 4 days

  Method and collection

  Method

  See individual test components.

  Collection

  Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze plasma within four hours of blood collection and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit seperate frozen specimens for each test requested.

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Single-analyte tests

• ADAMTS13 Activity with Reflex to Inhibitor 14532

  Plasma · Fasting not stated · Turnaround Set up: Sun-Thurs

  Method and collection

  Method

  Immunoassay (IA)

  Collection

  Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Freeze immediately and transport on dry ice.

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