Bordetella pertussis DNA and Bordetella parapertussis DNA

Single-analyte tests

• Bordetella pertussis/parapertussis DNA, Qualitative, Real-Time PCR 11365

  Swab · Fasting not stated · Turnaround Set up: Daily; Report available: 3 days

  Method and collection

  Method

  Real-Time Polymerase Chain Reaction (PCR) This test detects but does not distinguish between B. Pertussis and B. Holmesii.

  Collection

  Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial. Nasopharyngeal swab: Collection kits using Amies liquid, M4, VCM/UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for a few seconds for optimal collection. Collection of both nostrils is recommended but not required.

  Quest Diagnostics catalog page

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