Biomarkers · Chemical
Factor VIII Act, Chromogenic
- Primary unit
- %
- LOINC codes
- 3211-0
- Tests that measure Factor VIII Act, Chromogenic
- Labcorp 1
Tests
Check the catalog code, specimen, method and preparation for every test that measures Factor VIII Act, Chromogenic. Select up to five tests to compare them side by side.
Labcorp 1 test
Single-analyte tests
Factor VIII Activity, Chromogenic 086295
Plasma · Fasting not stated · Turnaround 3 - 5 days
Labcorp catalog pageMethod and collection
- Method
- The FVIII chromogenic substrate assay measures the FVIII-dependent activation of FX using purified bovine coagulation factors.1,2 This test consists of two steps. In the first step, patient plasma is added to a reaction mixture containing FIXa, FX, calcium ions, phospholipids and trace amounts of thrombin. Thrombin triggers the activation of FVIII and the subsequent FIXa-mediated activation of FX. FXa production is proportional to the concentration of FVIII in the plasma samples. In the second step, the amount of FXa produced is quantified using a chromogenic peptide substrate that binds selectively to FXa.3
- Preparation
- Ideally the patient should not be on anticoagulant therapy. Avoid warfarin (Coumadin®) therapy for two weeks prior to the test and heparin, direct Xa and thrombin inhibitor therapies for about three days prior to testing. Do not draw from an arm with a heparin lock or heparinized catheter.
- Collection
- Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.45 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.46,47 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.48,49 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested . Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.
- Reference notes
- 49–126%
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1 test: codes, specimens, methods, preparation and reference intervals.
LOINC 3211-0
Regenstrief Institute codes that identify the same result across labs.
Search ClinicalTrials.gov for Factor VIII Act, Chromogenic
Registered studies that measure Factor VIII Act, Chromogenic, on the U.S. National Library of Medicine registry.
Verify these ranges and sources
We compile this page from named laboratory catalogs, guidelines and studies. Labs calculate reference intervals from the people they test, for one assay method and one population group, so an interval describes that population rather than an optimal target.
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