Hepatitis B Virus Drug Resistance Genotype and BCPPrecore Mutations

Panels that include this biomarker

• Hepatitis B Virus Drug Resistance, Genotype, and BCP/Precore Mutations 10529

  2 analytes · Plasma · Fasting not stated · Turnaround Set up: Mon, Thurs

  Method and collection

  Method

  Polymerase Chain Reaction (PCR) • Sequencing

  Collection

  ⁠⁠⁠⁠⁠⁠⁠Plasma: Collect blood in sterile tube containing EDTA anticoagulant; either 0.15% solution v/v final EDTA K3 (standard EDTA tube) or 9 mg spray-dried EDTA K2 (plasma preparation tube or PPT tube with plasma separator-gel, preferred). Store whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic transport tube and store at -18° C or colder. If blood is collected in a PPT tube, centrifuge preferably within 2 hours of collection as before but it is not necessary to transfer plasma to aliquot tubes. Following centrifugation, a gel barrier maintains separation of plasma from cellular components during specimen transport and storage. The PPT is plastic and hence plasma can be stored and shipped frozen in the original tube. Avoid repeated freezing and thawing of specimen. Ship frozen. Serum: Collect blood in sterile tube with no anticoagulants; plastic Serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile plastic transport tube and store refrigerated or frozen. Ship frozen. Note: This test can only be performed reliably on specimens with a viral load of at least 600 IU/mL. For unknown viral loads order test code 16694.

  Reference notes

  See Laboratory Report

  Quest Diagnostics catalog page

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