Myeloperoxidase

Single-analyte tests

• Myeloperoxidase (MPO) 123006

  Plasma · Fasting not stated · Turnaround 2 - 4 days

  Method and collection

  Method

  Latex enhanced immunoturbidimetric method

  Collection

  Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake). Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C. If a refrigerated centrifuge is not available, chill the centrifuge carrier in the refrigerator before use until cold. Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C for five days. If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation. Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.

  Reference notes

  • Low CVD Risk: <470 pmol/L • Moderate Risk: 470–539 pmol/L • High Risk: >539 pmol/L

  Labcorp catalog page

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Single-analyte tests

• Cardio IQ® Myeloperoxidase (MPO) 92814

  Plasma · Fasting not stated · Turnaround Set up: Mon-Fri

  Method and collection

  Method

  Turbidimetric Immunoassay

  Collection

  1. Draw and gently invert 8 to 10 times. 2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature. 3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer. Note: This ensures that the buffy coat and red cells remain undisturbed. 4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma and cap tightly. Discard original tube. 5. Store transport tube refrigerated at 2-8°C until ready to ship. Store plasma at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box. Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.

  Reference notes

  <470 pmol/L

  Quest Diagnostics catalog page

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