Vitamin B6 Plasma

Single-analyte tests

• Micronutrient, Vitamin B6, Plasma 10193

  Plasma · Fasting required · Turnaround Set up: Sun-Fri; Report available: 1-4 days

  Method and collection

  Method

  Chromatography/Mass Spectrometry

  Preparation

  Overnight fasting is required

  Collection

  Patients should be 18 years of age or greater. Separate cells within 6 hours by centrifugation for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen.

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• Vitamin B6, Plasma 926

  Plasma · Fasting required · Turnaround Set up: Sun-Fri (8:00 a.m.); Report available: 1-4 days

  Method and collection

  Method

  Chromatography/Mass Spectrometry

  Preparation

  Overnight fasting is required

  Collection

  Separate cells within 24 hours by centrifugation for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen.

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Panels that include this biomarker

• Micronutrients, B-Vitamin Panel 10195

  7 analytes · Serum · Fasting required

  Method and collection

  Method

  Immunoassay (IA) • Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

  Preparation

  Overnight fasting is required. Avoid vitamin supplements for 24 hours prior to collection.

  Collection

  Patients should be 18 years of age or greater. Folate & Vitamin B12: Transfer 2 mL (1 mL min) serum into plastic transport tube, ship frozen. Vitamin B2, B3, B5: Transfer 2 mL (1 mL min) serum into an amber vial (or wrap with aluminum foil), ship frozen. Vitamin B6: Transfer 3 mL (1 mL min) EDTA plasma into an amber vial (or wrap with aluminum foil), ship frozen. ⁠⁠⁠⁠⁠⁠⁠Separate cells within 24 hours by centrifugation for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen. Vitamin B1: Transfer 3 mL (1 mL min) EDTA whole blood into an amber vial (or wrap with aluminum foil), ship frozen. Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Remove serum or plasma from cells within 4 hours of collection.

  Reference notes

  ≥18 years Folate | >5.4 ng/mL Vitamin B1 (Thiamine) | 78-185 nmol/L Vitamin B2 | 6.2-39.0 nmol/L Vitamin B3 | ≤110 ng/mL Vitamin B5 | <275 ng/mL Vitamin B6 | 5.1-54.8 ng/mL Vitamin B12 | 200-1100 ng/mL Reference range not available for individuals <18 years for this micronutrient test.

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Single-analyte tests

• Vitamin B6, Plasma 004655

  Plasma · Fasting not stated · Turnaround 4 - 6 days

  Method and collection

  Method

  Liquid chromatography-tandem mass spectrometry (LC/MS-MS) This test measures pyridoxal 5-phosphate, the biologically active form of vitamin B6.

  Collection

  Collect blood by venipuncture into a lavender-top tube containing EDTA and mixed immediately by gentle inversion at least six times to ensure adequate mixing. The plasma must be separated and protected from light in an amber transport tube with amber stopper. Specimens should be stored refrigerated or frozen immediately and maintained at temperature during shipping and at the testing facility. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

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