Euglobulin Clot Lysis Time

Single-analyte tests

• Euglobulin Clot Lysis Time (ECLT) 086145

  Plasma · Fasting not stated · Turnaround 6 - 8 days

  Method and collection

  Method

  The basic method of euglobulin preparation involves dilution and acidification of chilled plasma to form a precipitate, which is collected and re-dissolved in higher pH buffer containing calcium. Clotting occurs rapidly and clot lysis takes place over several hours. Results are reported as lysis time.

  Preparation

  Do not draw from an arm with a heparin lock or heparinized catheter.

  Collection

  Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.40 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.41,42 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (i.e., "butterfly"), in which case a discard tube should be used.43,44 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Double centrifugation is required for this test to ensure the removal of platelets that contain fibrinolysis factors.45,46 Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.

  Reference notes

  277–454 minutes

  Labcorp catalog page

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Panels that include this biomarker

• Fibrinolysis Profile 117310

  7 analytes · Plasma · Fasting not stated · Turnaround 5 - 8 days

  Method and collection

  Method

  See individual tests.

  Collection

  Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.27 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.28,29 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.30,31 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Double centrifugation is required for this test to ensure the removal of platelets that contain fibrinolysis factors.32,33 Centrifuge and carefully remove the plasma using a plastic transfer pipette, being careful not to disturb the cells. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.

  Labcorp catalog page

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Single-analyte tests

• Euglobulin Clot Lysis Time 462

  Plasma · Fasting not stated · Turnaround Set up: Wed (a.m.)

  Method and collection

  Method

  Clot Dissolution

  Collection

  Specimen collection container is blue-top (3.2% sodium citrate) tube. To avoid release of plasminogen activator, do not massage vein vigorously, pump fist excessively or leave tourniquet in place for a prolonged period. Centrifuge within 30 minutes after collection to get platelet-poor plasma and freeze on dry ice. Ship specimens frozen on dry ice. Keep samples in a -10 to -30° C freezer if they cannot be shipped promptly. Prohibit exercise prior to drawing sample.

  Reference notes

  >60 min

  Quest Diagnostics catalog page

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Panels that include this biomarker

• Fibrinolysis Comprehensive Panel 90923

  9 analytes · Plasma · Fasting required

  Method and collection

  Method

  Chromogenic Assay • Clot Dissolution • Hemagglutination (HA) • Immunoassay (IA) • Latex Agglutination (LA)

  Preparation

  Morning fasting is required. Since lipemia may interfere with clotting endpoint detection, a two-hour fast is recommended.

  Collection

  Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice. To avoid release of plasminogen activator, do not massage vein vigorously, pump fist excessively or leave tourniquet in place for a prolonged period. Centrifuge within 30 minutes after collection to get platelet-poor plasma and freeze on dry ice. Prohibit exercise prior to drawing sample.

  Reference notes

  See Laboratory Report

  Quest Diagnostics catalog page

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