Gonococcus Prequot, NAA, Cervix

Panels that include this biomarker

• Chlamydia/Gonococcus − Client-provided Aliquot 186200

  2 analytes · Swab · Fasting not stated · Turnaround 2 - 3 days

  Method and collection

  Method

  Nucleic acid amplification (NAA)

  Collection

  ThinPrep® preservative vial processing: ThinPrep® instructions for removal of material are specifically listed in the ThinPrep® 2000 and ThinPrep® 3000 System Operator's manual Addenda. These instructions include the following statement. “Good laboratory practices should be followed to avoid introducing contaminants into either the PreservCyt® sample vial (ThinPrep® vial) or the aliquot. It is recommended to use powder-free gloves and an individually wrapped, disposable pipetting device with an aerosol barrier tip that is sized appropriately for the volume being withdrawn and dispensed. You should not use serological pipettes. In order to minimize the potential for cross contamination, aliquot removal should be performed in an appropriate location outside an area where amplification is performed.” LabCorp uses the following procedure and believes it is consistent with the previous instructions as well as with the amplified assay that will be performed on the specimen to be sent. Note: For testing, aliquots must be transferred to Aptima® specimen transport tubes or Aptima® Unisex specimen transport tubes (both referred to as “Aptima® tube” in the following procedure) from ThinPrep® vials within seven days of specimen collection when ThinPrep® vials are stored at 15°C to 30°C. Aliquot Removal Procedure 1. Put on clean gloves. 2. Wipe down work surfaces and pipettors with 1:1 bleach:water solution. 3. Allow bleach to contact work surfaces and pipettors for at least one minute and then follow with a water rinse. 4. Cover the bench with clean, plastic-backed absorbent laboratory bench covers. 5. Retrieve ThinPrep® vials that are to have aliquots removed. 6. In the specimen transfer area, place a test tube rack containing a sufficient number of Aptima® tubes to the number of ThinPrep® Pap specimens being tested for Ct/Ng. 7. Label each Aptima® tube with the appropriate specimen label. 8. Put on clean gloves before proceeding to specimen transfer. 9. Loosen the cap on the Aptima® tube. Be sure not to touch the pierceable foil cap of the Aptima® tube. 10. Vortex the ThinPrep® vial for 8 to 12 seconds at high speed. Caution: The desired aliquot must be removed immediately after vortexing the vial to ensure homogeneity of the sample. 11. Uncap the vial and place it on the work surface with the threads facing up. 12. Using a pipetting device with an aerosol barrier tip, immediately withdraw a 1 mL aliquot from the ThinPrep® vial. 13. Uncap the Aptima® tube and retain the cap in your hand. 14. Carefully dispense the aliquot into the appropriately labeled Aptima® tube. 15. Recap the Aptima® tube tightly. Gently invert the tube two to three times to ensure complete mixing of the specimen. 16. Dispose of the pipette tip into a biohazard bag containing absorbent material soaked with bleach. 17. Recap the ThinPrep® Liquid Pap specimen for Pap smear processing. 18. Store the aliquot in Aptima® tube at 15°C to 30°C for transport to LabCorp. 19. Put on clean gloves and repeat steps 8 to 18 with subsequent specimens. To reduce the chance of contaminating other specimens, work with one ThinPrep® Liquid Pap specimen at a time. 20. Remember that prior to processing for Pap smear in the ThinPrep® instrumentation, a volume of PreservCyt® equal to that removed for testing must be added back to the vial.

  Reference notes

  Negative; no Chlamydia trachomatis or Neisseria gonorrhoeae detected.

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• Gynecologic Pap Test (Image-guided), Liquid-based Preparation and Chlamydia/Gonococcus , NAA 196402

  2 analytes · Cervical cells collected by one of the methods described below. · Fasting not stated · Turnaround 2 - 5 days

  Method and collection

  Method

  Image-guided liquid-based Pap test; nucleic acid amplification (NAA) ( Chlamydia/Gonococcus )

  Preparation

  Patient should avoid douches 48 to 72 hours prior to examination. Specimen should not be collected during or shortly after menstrual period.

  Collection

  ThinPrep® Vial − Broom or Brush/Spatula Broom-like collection technique: Obtain a sample from the cervix using a broom-like device by inserting the brush portion into the cervical os and then rotate the brush five times. Rinse the collection device in the PreservCyt® solution by pushing the brush into the bottom of the vial 10 times, forcing the bristles to bend apart to release the cervical material. As a final step, twirl the brush between the thumb and forefinger vigorously to release additional cellular material. Discard the collection device. Tighten the cap on the ThinPrep® vial so that the torque line on the cap passes the torque line on the vial. Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush ¼ to ½ turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial. Optional Dedicated Specimen for Chlamydia and Gonococcus: Use the Gen-Probe® Aptima® swab collection kit. ( Note: Do not use the Gen-Probe® PACE DNA probe collection kit.) Clean the cervix using the larger, white-shafted swab supplied in the Gen-Probe® Aptima® swab collection kit and discard. Insert the smaller, blue-shafted swab into the cervix and rotate for 10 to 30 seconds to ensure good sampling. Carefully withdraw the blue-shafted swab, avoiding contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Break the swab shaft at the scoreline, using care to avoid splashing contents. Recap the swab specimen transport tube tightly.

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• Gynecologic Pap Test (Image-guided), Liquid-based Preparation and Chlamydia/Gonococcus , NAA With Reflex to Cobas® High-risk HPV With HPV Genotypes 16 and 18 When ASC-U, ASC-H, LSIL, HSIL, AGUS 196245

  2 analytes · Cervical cells collected by one of the methods described below. · Fasting not stated · Turnaround 3 - 6 days

  Method and collection

  Method

  Image-guided liquid-based Pap test; nucleic acid amplification (NAA)

  Preparation

  Patient should avoid douches 48 to 72 hours prior to examination. Specimen should not be collected during or shortly after menstrual period.

  Collection

  Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush ¼ to ½ turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial. Optional Dedicated Specimen for Chlamydia and Gonococcus: Use the Gen-Probe® Aptima® swab collection kit. Clean the cervix using the larger, white-shafted swab supplied in the Gen-Probe® Aptima® swab collection kit and discard. Insert the smaller, blue-shafted swab into the cervix and rotate for 10 to 30 seconds to ensure good sampling. Carefully withdraw the blue-shafted swab, avoiding contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Break the swab shaft at the scoreline, using care to avoid splashing contents. Recap the swab specimen transport tube tightly.

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• Gynecologic Pap Test (Image-guided), Liquid-based Preparation and Chlamydia/Gonococcus , NAA With Reflex to High-risk HPV (Cobas®) When ASC-U 196315

  2 analytes · Cervical cells collected by one of the methods described below. · Fasting not stated · Turnaround 2 - 6 days

  Method and collection

  Preparation

  Patient should avoid douches 48 to 72 hours prior to examination. Specimen should not be collected during or shortly after menstrual period.

  Collection

  Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush 1/4 to 1/2 turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial. Optional Dedicated Specimen for Chlamydia and Gonococcus: Use the Gen-Probe® Aptima® swab collection kit. ( Note: Do not use the Gen-Probe® PACE DNA probe collection kit.) Clean the cervix using the larger, white-shafted swab supplied in the Gen-Probe® Aptima® swab collection kit and discard. Insert the smaller, blue-shafted swab into the cervix and rotate for 10 to 30 seconds to ensure good sampling. Carefully withdraw the blue-shafted swab, avoiding contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Break the swab shaft at the scoreline, using care to avoid splashing contents. Recap the swab specimen transport tube tightly.

  Labcorp catalog page

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• Gynecologic Pap Test (Image-guided), Liquid-based Preparation and Chlamydia/Gonococcus , NAA With Reflex to High-risk HPV (Cobas®) With HPV Genotypes 16 and 18 When ASC-U 196230

  2 analytes · Cervical cells collected by one of the methods described below. · Fasting not stated · Turnaround 2 - 6 days

  Method and collection

  Method

  Image-guided liquid-based Pap test; nucleic acid amplification (NAA) for Chlamydia/Gonococcus and HPV

  Preparation

  Patient should avoid douches 48 to 72 hours prior to examination. Specimen should not be collected during or shortly after menstrual period. Excessive use of lubricating jelly will interfere with cytologic examination.

  Collection

  Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush 1/4 to 1/2 turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial. Optional Dedicated Specimen for Chlamydia and Gonococcus: Use the Gen-Probe® Aptima® swab collection kit. ( Note: Do not use the Gen-Probe® PACE DNA probe collection kit.) Clean the cervix using the larger, white-shafted swab supplied in the Gen-Probe® Aptima® swab collection kit and discard. Insert the smaller, blue-shafted swab into the cervix and rotate for 10 to 30 seconds to ensure good sampling. Carefully withdraw the blue-shafted swab, avoiding contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Break the swab shaft at the scoreline, using care to avoid splashing contents. Recap the swab specimen transport tube tightly.

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