Plasminogen Act Inhibitor-1,Ag

Single-analyte tests

• Plasminogen Activator Inhibitor (PAI-1) Ag 36555

  Plasma · Fasting not stated · Turnaround Set up: Tues (p.m.); Report available: 5 days

  Method and collection

  Method

  Enzyme Immunoassay (EIA)

  Reference notes

  4-43 ng/mL

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Panels that include this biomarker

• Fibrinolysis Comprehensive Panel 90923

  9 analytes · Plasma · Fasting required

  Method and collection

  Method

  Chromogenic Assay • Clot Dissolution • Hemagglutination (HA) • Immunoassay (IA) • Latex Agglutination (LA)

  Preparation

  Morning fasting is required. Since lipemia may interfere with clotting endpoint detection, a two-hour fast is recommended.

  Collection

  Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice. To avoid release of plasminogen activator, do not massage vein vigorously, pump fist excessively or leave tourniquet in place for a prolonged period. Centrifuge within 30 minutes after collection to get platelet-poor plasma and freeze on dry ice. Prohibit exercise prior to drawing sample.

  Reference notes

  See Laboratory Report

  Quest Diagnostics catalog page

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Single-analyte tests

• Plasminogen Act Inhibitor-1,Ag 146790

  Plasma · Fasting not stated · Turnaround 4 - 7 days

  Method and collection

  Method

  Enzyme-linked immunosorbent assay (ELISA)

  Collection

  Citrated plasma samples should be collected by double centrifugation. Platelet-poor plasma is needed. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp No. 49482). Freeze immediately and maintain frozen until tested. Print and use the Specimen Collection Bulletin as a tube-filling guide.

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