Biomarker Lens

Common biomarkers

  • Hemoglobin
  • Glucose
  • ALT
  • Apolipoprotein B
  • C-Reactive Protein, Cardiac
  • Ferritin
  • Alb/Creat Ratio, Urine
  • Alkaline Phosphatase
  • FSH
  • T4,Free(Direct)

Biomarkers · Chemical

Testosterone,Free

A testosterone levels test measures the amount of the hormone testosterone in a sample of your blood. Hormones are chemical messengers in your bloodstream that control the actions of certain cells or organs.

Testosterone Levels Test, MedlinePlus, National Library of Medicine

Primary unit
ng/dL
LOINC codes
2991-8
Tests that measure Testosterone,Free
Labcorp 11, Quest Diagnostics 2

Ranges

Each lab publishes a reference range for the population groups it tests, usually the middle 95% of results. These intervals describe that population, not an optimal or healthy target. How ranges differ

Quest Diagnostics, Testosterone Free, Calculation 18944

Provider contextMale (pg/mL)Female (pg/mL)
<1 YearNot establishedNot established
1-11 Years≤1.3≤1.5
12-13 Years≤64.0≤1.5
14-17 Years4.0-100.0≤3.6
18-69 Years46.0-224.00.2-5.0
70-89 Years6.0-73.00.3-5.0
>89 YearsNot establishedNot established

Quest Diagnostics test catalog, 18944

Tests

Check the catalog code, specimen, method and preparation for every test that measures Testosterone,Free. Select up to five tests to compare them side by side.

Labcorp 11 tests

Single-analyte tests

  • Testosterone, Free, Direct 144980

    Serum · Fasting not stated · Turnaround 4 - 6 days

    Method and collection
    Method
    Direct analog enzyme immunoassay (EIA)
    Collection
    If a red-top tube is used, transfer separated serum to a plastic transport tube.
    Reference notes
    See table. Gender/Age (y) Range (pg/mL) Male 0 to 19 Not established 20 to 29 9.3−26.5 30 to 39 8.7−25.1 40 to 49 6.8−21.5 50 to 59 7.2−24.0 >59 6.6−18.1 Female 0 to 19 Not established >19 0.0−4.2
    Labcorp catalog page

Panels that include this biomarker

  • Testosterone, Free, Direct With Total Testosterone 140103

    2 analytes · Serum · Fasting not stated · Turnaround 4 - 7 days

    Method and collection
    Method
    Free: Direct analog enzyme immunoassay (EIA); Total: electrochemiluminescence immunoassay (ECLIA)
    Collection
    If a red-top tube is used, transfer separated serum to a plastic transport tube.
    Labcorp catalog page
  • Testosterone, Free, Direct With Total Testosterone, LC/MS-MS 070195

    2 analytes · Serum · Fasting not stated · Turnaround 4 - 6 days

    Method and collection
    Method
    Free: Direct analog enzyme immunoassay (EIA); Total: liquid chromatography/tandem mass spectrometry (LC/MS-MS)
    Collection
    Serum should be transferred from cells within one hour of collection and transferred to a plastic transport tube.
    Labcorp catalog page
  • Testosterone, Free, Equilibrium Ultrafiltration With Total Testosterone 081786

    3 analytes · Serum · Fasting not stated · Turnaround 2 - 4 days

    Method and collection
    Method
    Free: equilibrium ultrafiltration; total: electrochemiluminescence immunoassay (ECLIA)
    Collection
    Separate serum from cells. If a red-top tube is used, transfer separated serum to a plastic transport tube.
    Reference notes
    See tables.1 Free Testosterone Age | Male (ng/dL) | Female (ng/dL) Cord blood | 0.50−2.20 | 0.40−1.60 1 to 15 d | 0.15−3.10 | 0.05−0.25 1 to 2 m | 0.33−0.80 | 0.01−0.13 3 to 5 m | 0.07−1.40 | 0.03−0.11 6 to 7 m | 0.04−0.48 | 0.02−0.06 6 to 9 y | 0.01−0.32 | 0.01−0.09 10 to 11 y | 0.06−0.57 | 0.10−0.52 12 to 14 y | 0.14−15.60 | 0.10−0.52 15 to 17 y | 8.00−15.90 | 0.10−0.52 Adults | 5.00−21.00 | 0.10−0.85 Percentage of Total Testosterone Age | Male (%) | Female (%) Cord blood | 2.00−4.40 | 2.00−3.90 1 to 15 d | 0.90−1.70 | 0.80−1.50 1 to 2 m | 0.40−0.80 | 0.40−1.10 3 to 5 m | 0.40−1.10 | 0.50−1.00 6 to 7 m | 0.40−1.00 | 0.50−0.80 6 to 9 y | 0.90−1.70 | 0.90−1.40 10 to 11 y | 1.00−1.90 | 1.00−1.90 12 to 14 y | 1.30−3.00 | 1.00−1.90 15 to 17 y | 1.80−2.70 | 1.00−1.90 Adults | 1.50−4.20 | 0.50−2.80
    Labcorp catalog page
  • Testosterone, Free, Equilibrium Ultrafiltration With Total Testosterone, LC/MS-MS 070038

    3 analytes · Serum · Fasting not stated · Turnaround 4 - 6 days

    Method and collection
    Method
    Free: equilibrium ultrafiltration; total: liquid chromatography/tandem mass spectrometry (LC/MS-MS)
    Collection
    Serum should be transferred from cells within one hour of collection and transferred to a plastic transport tube.
    Reference notes
    See table.1 Free Testosterone Age | Male (ng/dL) | Female (ng/dL) Cord blood | 0.50−2.20 | 0.40−1.60 1 to 15 d | 0.15−3.10 | 0.05−0.25 1 to 2 m | 0.33−0.80 | 0.01−0.13 3 to 5 m | 0.07−1.40 | 0.03−0.11 6 to 7 m | 0.04−0.48 | 0.02−0.06 6 to 9 y | 0.01−0.32 | 0.01−0.09 10 to 11 y | 0.06−0.57 | 0.10−0.52 12 to 14 y | 0.14−15.60 | 0.10−0.52 15 to 17 y | 8.00−15.90 | 0.10−0.52 Adults | 5.00−21.00 | 0.10−0.85 Percentage of Total Testosterone Age | Male (%) | Female (%) Cord blood | 2.00−4.40 | 2.00−3.90 1 to 15 d | 0.90−1.70 | 0.80−1.50 1 to 2 m | 0.40−0.80 | 0.40−1.10 3 to 5 m | 0.40−1.10 | 0.50−1.00 6 to 7 m | 0.40−1.00 | 0.50−0.80 6 to 9 y | 0.90−1.70 | 0.90−1.40 10 to 11 y | 1.00−1.90 | 1.00−1.90 12 to 14 y | 1.30−3.00 | 1.00−1.90 15 to 17 y | 1.80−2.70 | 1.00−1.90 Adults | 1.50−4.20 | 0.50−2.80
    Labcorp catalog page
  • Testosterone, Free, Mass Spectrometry/Equilibrium Dialysis (Endocrine Sciences) 500726

    3 analytes · Serum · Fasting not stated · Turnaround 6 - 10 days

    Method and collection
    Method
    Testosterone: high-pressure liquid chromatography (HPLC)/tandem mass spectrometry; free testosterone: equilibrium dialysis
    Collection
    Serum must be separated from cells within 45 minutes of venipuncture. Send serum in a plastic transport tube. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested .
    Reference notes
    See tables. Testosterone Expected Values Age Male (ng/dL) Female (ng/dL) Premature Infants 26 to 28 wk, day 4 59−125 5−16 31 to 35 wk, day 4 37−198 5−22 Full-term Infants Newborns, 1 to 7 m 75−400 20−64 Prepubertal Children 1 to 10 y <2.5−10 1 to 9 y <2.5-10 Adults >18 y 264−916 Premenopausal 10−55 Postmenopausal 7−40 Tanner Stage - Pubertal Ages Tanner Stage Male Female Age (y) Testosterone (ng/dL) Age (y) Testosterone (ng/dL) I <9.8 <2.5−10 <9.2 <2.5−10 II 9.8−14.5 18−150 9.2−13.7 7−28 III 10.7−15.4 100−320 10.0−14.4 15−35 IV 11.8−16.2 200−620 10.7−15.6 13−32 V 12.8−17.3 350−970 11.8−18.6 20−38 Free Testosterone Expected Values Age Male Female % Free pg/mL % Free pg/mL Full-term Infants 1 to 15 d 0.9−1.7 1.5−31 0.8−1.5 0.5−2.5 1 to 2 m 0.4−0.8 3.3−18 0.4−1.1 0.1−1.3 3 to 4 m 0.4−1.1 0.7−14 0.5−1.0 0.3−1.1 5 to 6 m 0.4−1.0 0.4−4.8 0.5−0.8 0.2−0.6 Prepubertal Children 1 to 10 y 0.4−0.9 0.15−0.6 Same as males Puberty: Comprehensive values for free testosterone by dialysis for both males and females throughout puberty are currently unavailable. Male Female % Free pg/mL % Free pg/mL Adults 1.5−3.2 52−280 0.8−1.4 1.1−6.3
    Labcorp catalog page

Quest Diagnostics 2 tests

Single-analyte tests

  • Testosterone Free, Calculation 18944

    Serum · Fasting not stated · Turnaround Set up: Mon-Fri

    Method and collection
    Method
    Chromatography/Mass Spectrometry • Calculation (CALC) • Immunochemiluminescence Assay (ICMA)
    Collection
    Specify age and sex on test request form. Red-top tube (no gel): Allow blood to clot at room temperature. Centrifuge to separate the serum from the cells and immediately pour serum into a plastic transport tube. Serum separator tube: Allow blood to clot at room temperature, centrifuge, and remove serum from the gel immediately (not to exceed 48 hours) by pouring serum into a plastic transport tube.
    Quest Diagnostics catalog page

Panels that include this biomarker

  • Testosterone, Free (Dialysis), Total (MS) and Sex Hormone Binding Globulin 37073

    3 analytes · Serum · Fasting not stated

    Method and collection
    Method
    Chromatography/Mass Spectrometry • Equilibrium Dialysis • Calculation (CALC) • Immunoassay (IA)
    Collection
    Specify age and sex on test request form. Red-top tube (no gel): Allow blood to clot at room temperature. Centrifuge to separate the serum from the cells and immediately pour serum into a plastic transport tube. Serum separator tube: Allow blood to clot at room temperature, centrifuge, and remove serum from the gel immediately (not to exceed 48 hours) by pouring serum into a plastic transport tube. Administration of STRENSIQ may interfere in certain assays and may falsely elevate values. For patients receiving STRENSIQ, consideration should be given to using alternate methods.
    Reference notes
    See Laboratory Report
    Quest Diagnostics catalog page

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Sources

Verify these ranges and sources

We compile this page from named laboratory catalogs, guidelines and studies. Labs calculate reference intervals from the people they test, for one assay method and one population group, so an interval describes that population rather than an optimal target.

Interpret your own results with a licensed clinician, or track your lab history and health goals in Aumnivida.

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